Molecular Biology Products
yeast research tools
A selection of yeast research tools and accessories are offered to aid researchers in a wide variety of applications using yeast.
EZ Yeast™ Plasmid Prep
The EZ Yeast™ Plasmid Prep kit is designed for the rapid isolation of 2μ plasmids from yeast patches or yeast grown in small liquid cultures. The kit is adapted from the alkaline lysis of E. coli, by providing modified alkaline lysis buffers and our LongLife™ Zymolyase®, a highly stable enzyme.The EZ Yeast™ Plasmid Prep kit makes it possible to isolate high yield yeast plasmid without the use of glass beads, phenol, or repeated vortexing.
LongLife™ Zymolyase®, a high performance lytic enzyme preparation, efficiently releases plasmids with a yield of up to 0.1-0.3ng for most 2-based plasmids from a 1.5ml culture (1 prep). EZ Yeast™ Plasmid Prep is suitable even for low copy number yeast plasmids.
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Fast Yeast Transformation™
Fast Yeast Transformation™ kit is a rapid single step yeast transformation kit that takes less than 10 minutes to prepare competent yeast cells. The competent yeast cells can be used immediately or frozen for later use. This method is suitable for both circular and linear plasmid transformations. The protocol involves simply suspending yeast cells in the supplied Competent Buffer and the cells are then ready to receive transforming DNA. Introduce DNA to the competent cells and incubate for transformation.
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A high performance LongLife™ Zymolyase® preparation is supplied in a ready-to-use solution form. The enzyme contains high yeast lytic activity with low non-specific activity. Suitable for yeast cell lysis, spheroplast preparation, glucan hydrolysis and more.
The Yeast Geno-DNA-Template™ extraction kit isolates high quality genomic DNA from yeast. The kit is based on a two-step lysis of yeast cells, using LongLife™ Zymolyase® and LongLife™ Proteinase K, followed by the removal of proteins and other cellular impurities by precipitation and centrifugation.
The clean, genomic DNA is collected by precipitation and the high yield of extracted DNA has an average 100kb length and has A(260/280) of 1.8-2.0.
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