Molecular Biology Products
A selection of RNA products for the isolation of RNA and the detection, decontamination and digestion of RNase.
RNaseOUT™ is uniquely formulated to destroy and remove RNases on contact. Simply spray and rinse. RNaseOUT™ is non-toxic and residue free. RNaseOUT™ allows common equipment to be safely and confidently used for all RNA work.
RNaseOUT™ is supplied in 250ml easy to apply spray bottles. Simply spray on items or area to decontaminate and rinse. Spraying prevents waste and is designed to uniformly cover the application area.
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GET™ Total RNA
The GET™ Total RNA kit isolates total RNA from contaminating DNA, proteins, and nucleases using our GET™ RNA Spin Columns. The <60-minute protocol is simple; after homogenization, RNA is bound to the GET™ RNA Spin Columns and washed.
The protocol provides an option to remove contaminating DNA with a single DNase treatment. Finally RNA is eluted from the column. The eluted RNA is ready for any procedure including Northern/slot/dot blots, reverse transcription or RNase protective assays.
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The TOTAL Arrest™ RNA kit isolates total RNA from contaminating DNA, proteins, and nucleases using our proprietary binding matrix, pinkRESIN™. The absence of phenol/chloroform extractions make the Total Arrest™ RNA kit one of the safest methods for isolating and purifying high quality RNA. The 60 minute protocol (micro kit) is simple; after homogenization, RNA is bound to pinkRESIN™ and washed.
The protocol provides an option to remove contaminating DNA with a single DNase treatment. Finally RNA is eluted from pinkRESIN™. The eluted RNA is ready for further applications, including Northern and dot blots, reverse transcription or RNase protective assays. The kit is supplied in two formats, the Micro for 50 preps (10-50mg tissue/ prep) and Large for 10 preps (100-500mg tissue/ prep).
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Arrest™ Extraction Buffer
Arrest™ Extraction Buffer is an optimized combination of various chaotropic agents and RNase inhibitors, which inhibits RNase in 5-10 seconds. Arrest™ Extraction Buffer may be used in conjunction with any RNA extraction method, including extractions based on phenol, chloroform and other organic solvents and detergents.
The quick 10 minute single step protocol isolates high quality total RNA from most species and tissues. Protocol involves homogenization and lysis of samples in Arrest™ Buffer. After removing cellular debris, pure RNA is precipitated from the supernatant with ethanol.
With RNase-DETECT™, 10µl of test solution is added to our calibrated RNA substrate vial, incubated, and the result viewed after 10 minutes by agarose electrophoresis.
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