Molecular Biology Products | Genomic DNA Purification
megalong™ genomic dna purification
The majority of genomic DNA extraction methods involve numerous physical manipulations, including mixing, pipetting, shaking, binding to resin, elution, which results in sheared DNA that may not be suitable for further analysis.
MegaLong™ isolates high molecular weight genomic DNA from a variety of samples, including animal tissues, cultured cells, whole blood, bacterial and yeast. MegaLong™ uses Genomic Tube-O-DIALYZER™, a unique, patented micro dialysis device with a 0.45μm membrane that minimizes sample manipulation, one of the main reasons for DNA breakage. Nuclei are isolated under mild extraction conditions and genomic DNA is released by digestion of nuclear proteins with a highly active LongLife™ Proteinase K. The digestion is performed in the Tube-O-DIALYZER™ and after digestion the Tube-O-DIALYZER™ is inverted to dialyze away digested protein and other impurities leaving behind highly pure and fully hydrated genomic DNA.