phosphatase assays & accessories
G-Biosciences offers a phosphatase assay for the quantification of phosphatases and three phosphatase inhibitor cocktails to inhibit phosphatases and for phosphatase studies.
The Phosphatase Assay kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases. The Phosphatase Assay kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases, including alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases.
The phosphatases remove the phosphate group to generate p-nitrophenol, which is deprotonated under alkaline conditions to produce p-nitrophenolate that has strong absorption at 405nm. The kits components are sufficient for performing up to 1000 assays in 96-well plate format and easily adaptable to cuvettes or 384-well plates.
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PhosphataseArrest™ Phosphatase Inhibitor Cocktail
The PhosphataseArrest™ phosphatase inhibitor cocktails are ready-to-use 100X solutions that are simply added to your extraction buffers or samples.
A broad spectrum phosphatase inhibitor cocktail consisting of five phosphatase inhibitors that target serine/threonine specific, tyrosine specific and dual specificity phosphatases. PhosphataseArrest™ I is a stablized solution of sodium fluoride, sodium orthovanadate, sodium pyrophosphate, β-glycerophosphate & sodium molybdate.
A phosphatase inhibitor cocktail consisting of five phosphatase inhibitors that target acid, alkaline and tyrosine phosphatases. PhosphataseArrest™ II contains optimized concentrations of sodium fluoride, sodium tartrate, sodium orthovanadate, imidazole & sodium molybdate.
A phosphatase inhibitor cocktail consisting of three phosphatase inhibitors that target alkaline and serine/threonine phosphatases.PhosphataseArrest™ III is a stable, convenient 100X solution of cantharidin, bromotetramisole, and microcystin LR.
*NEW* PhosphataseArrest™ IV (Cat. #786-602)
For alkaline and serine/threoninephosphatases, consisting of cantharidin, p-bromotetramisole oxalate and calyculin A. 1ml 100X Solution.
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PhosphoQuant™ is specifically designed for quick and reliable determination of whether a purified protein is phosphorylated and the extent of phosphorylation. The assay is based on the alkaline hydrolysis of phosphates from seryl and threonyl residues in phosphoproteins and the subsequent quantification of the released phosphate with a Molybdate dye.
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